Animals, diets and experimental design
In brief, EuO extraction procedure was based on a water distillation process. Approximately 100 kg of Eucalyptus leaves were collected and put into 300 L steel barrel with 100 L of water. The barrel was then covered tightly with a lid which was connected with a pipe and cooling coil in a cooling device. At the end of cooling device, a plastic bottle was used for collecting aromatic fluid. The barrel was then boiled for approximately 5 h, and essential oils were then collected by separating the oils floating on the aromatic fluid.