Among the external factors that influence flowering induction, photoperiod is of high importance. Although sugarcane behaves as a short-day plant, successive long nights are also required to induce flowering ([4]). The ideal day length for flowering appears to be approximately 12 h 55 min and constitutes the day length adopted for most of the sugarcane cultivars grown worldwide ([5] [6]). Even at locations where the inductive photoperiod conditions occur, the emergence of inflorescences may not be uniform, revealing that the temperature is also important for flowering ([7]). It is believed that the minimum temperature rarely falls below 18°C and the maximum never exceed 32°C in areas with abundant flowering ([6] [8]). Moreover, temperatures below 21°C can delay growth and panicle emergence ([1]). The pollen grain viability is also affected by the temperature, mainly cold temperatures, resulting in pollen grains that are unviable; in fact, night temperatures below 15°C before or during flowering may cause anther abortion and male sterility ([5]).