It was alleged that one of the active compounds, andrographolide contained in the extract, was responsible for the lymphocytes cell proliferation. HPLC analysis on the extract showed that the extract was indeed contained andrographolide. Previous research reported that andrographolide was able to modulate the innate immune response by regulating both classical and alternative activation of macrophages, and regulate specific antibody production as well as antigen-specific IL-4 producing splenocytes (Wang et al. 2010). Ethanol extract and purified diterpen andrographolide of A. paniculata have been shown to induce significant stimulation of antibody and also stimulate macrophage migration, and in vitro proliferation of splenic lymphocytes. However, it was lower with purified andrographolides than with the ethanolic extract indicating presence of substances other than andrographolide that may be contributing towards immunostimulation (Puri et al.1993).