Eddie Gluskin makes his first appearance at the start of the game in the Underground Lab, being forced against his will to enter one of the glass spheres in the lab to undergo painstaking restraint and experiments. Waylon Park is there working on one of the computers for debugging to allow the aforementioned experiment. Before Eddie is finally restrained, he runs up to the glass begging for help, and exclaims that Waylon has the power to stop all of it before being forced again to enter one of the glass spheres.
Much later in the game where Waylon can pick up the "Kill Us" document, a voice can be heard on the other side of a blocked door screaming for help. Zooming with the camcorder reveals the voice is being followed by Eddie, who quickly disappears from the corridor. Waylon continues to the Vocational Block, where Eddie is once again seen when he pushes his face against the glass of one of the doors, smiling. He is notably scarred and acts out much like many Variants, though to an extremely much higher degree; he has misogynistic and transmisogynistic fantasies and mutilates other men to make them look like his ideal woman, including genital and chest mutilation, all in an attempt to create a "bride" for himself. This gives him his label as "The Groom."
During that time in the game, Eddie pursues Waylon. Waylon jumps down an elevator shaft to escape him, and gravely wounds his right leg while doing so, making him less mobile for the remainder of the game. With an injured leg, still attempting to escape Eddie, Waylon attempts to hide in a locker, but Eddie discovers this and drags the locker itself to another room - Eddie's "workshop," where he brutally mutilates others.
Eddie gasses the locker Waylon is inside of to "calm him down" while he cuts into another victim. Shortly after this, Eddie strips Waylon of all his clothes and places him on a table, preparing to cut into him with a buzzsaw, with Waylon's body placed so his genitals are aimed at the buzzsaw. Just before the spinning buzzsaw reaches Waylon's body, a Variant comes in and engages in a fight with Eddie. Waylon uses this distraction as an opportunity to escape through a broken window.