When considering the CLPP results of all carbon sources individually,
more detailed distinctions can be made. Fig. 5A shows a
linear discriminant analysis (LDA) of the Abs590nm values after 94 h
of incubation, using the data of harvesting at 16 and 53 days.
Additionally, group means for all sample types are depicted with a
probability ellipse (standard deviation, p ¼ 0.68), and the font size
of the carbon sources corresponds to the score of each source on a
SIMPER analysis distinguishing between AgNP-exposed and nonexposed
bulk soil samples at these harvesting points. Similarly to
the results in Fig. 3, the findings demonstrate that at these other in the non-exposed samples than in the AgNP-exposed
samples. However, at these harvesting points, the carbon use
pattern of AgNP-exposed rhizosphere was more different from that
of the non-exposed rhizosphere than was the case at harvesting
points 16 and 53 days. Nonetheless, this difference did not result
from a similar change in carbon use pattern as in the bulk soil, as
indicated by the SIMPER results in Fig. 5A and B. While C-sources
like D-xylose, phenylethylamine and 4-hydroxy benzoic acid
seemed to contribute the most to the difference between nonexposed
and AgNP-exposed bulk soil at harvesting points 16 and
53 days, it were mainly glycogen, alpha-cyclodextrin and itaconic
acid that distinguished non-exposed and AgNP-exposed rhizosphere
at 39 and 75 days. The general pattern shown by the LDAs
pointed out that no C-sources were strictly typical for any
sample type in general, and that differences were mainly caused
by generally lower C-oxidation values in AgNP-exposed samples,
compared to non-exposed samples.
When considering the CLPP results of all carbon sources individually,more detailed distinctions can be made. Fig. 5A shows alinear discriminant analysis (LDA) of the Abs590nm values after 94 hof incubation, using the data of harvesting at 16 and 53 days.Additionally, group means for all sample types are depicted with aprobability ellipse (standard deviation, p ¼ 0.68), and the font sizeof the carbon sources corresponds to the score of each source on aSIMPER analysis distinguishing between AgNP-exposed and nonexposedbulk soil samples at these harvesting points. Similarly tothe results in Fig. 3, the findings demonstrate that at these other in the non-exposed samples than in the AgNP-exposedsamples. However, at these harvesting points, the carbon usepattern of AgNP-exposed rhizosphere was more different from thatof the non-exposed rhizosphere than was the case at harvestingpoints 16 and 53 days. Nonetheless, this difference did not resultfrom a similar change in carbon use pattern as in the bulk soil, asindicated by the SIMPER results in Fig. 5A and B. While C-sourceslike D-xylose, phenylethylamine and 4-hydroxy benzoic acidseemed to contribute the most to the difference between nonexposedand AgNP-exposed bulk soil at harvesting points 16 and53 days, it were mainly glycogen, alpha-cyclodextrin and itaconicacid that distinguished non-exposed and AgNP-exposed rhizosphereat 39 and 75 days. The general pattern shown by the LDAspointed out that no C-sources were strictly typical for any
sample type in general, and that differences were mainly caused
by generally lower C-oxidation values in AgNP-exposed samples,
compared to non-exposed samples.
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