Biochemical parameters
All rats were anesthetized by diethyl ether after recording the final body weight. The blood samples of each animal were collected through cardiac puncture and serum was obtained by centrifuging blood samples at 4000 rpm at 25°C for 4 min and analyzed for assorted biochemical parameters. The obtained serum samples was preserved at −20°C. The total cholesterol (TC), high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, total triglyceride (TG), SGOT, SGPT and BUN was done through Artos - Versatile Clinical Chemistry Analyzer using standard kits (supplied by Swemed biomedicals pvt. ltd., India). Whole blood was collected separately (preserved with anticoagulating agents) for the estimation of glycosylated hemoglobin using standard kits (supplied by Swemed biomedicals pvt. ltd., India).