Molecular weight of okara polysaccharides was determined
by high-performance size-exclusion chromatography
(HPSEC), using a Waters 2695 HPLC system fitted with two
serially linked Waters Ultrahydrogel 250 and 2000 columns,
eluted with 0.1 M NaNO3 at a flow rate of 0.9 mL/min and
45 1C. The molecular weight was estimated by reference to a
calibration curve prepared using a Dextran series of different
molecular weights.