The analyses of mtDNA showed that all hybrid specimens possessed the haplotype of C. gibelio, i.e. the hybrids were descended
from C. gibelio maternally. Microsatellite analysis of 19 putative hybrid specimens revealed the presence of 12 alleles in the GF29
locus, 11 alleles in the MFW2 locus, 15 alleles in the MFW7 locus,
nine alleles in the J1 locus, and nine alleles in the J62 locus. For
each of the loci GF29, MFW2, MFW7 and J1, one allele corresponded
to C. carpio and the other to C. gibelio (Table 1) on the basis of either
alleles actually observed or size ranges described in Hänfling et al.
(2005) or Papoušek et al. (2008). Concerning locus J62, the alleles
in two hybrid specimens with a presumed origin from C. carpio
were slightly outside the observed size range, as already shown in
Šimková et al. (2013).