Each tissue derived from ten plants at the same growth stage were pooled together and surface sterilized with 70 % (v/v) ethyl alcohol for 1 min, 2%(v/v) NaOCl for 2 min, 95%(v/v) ethyl alcohol for 30 s and 30 % (v/v) H2O2 for 1 min, respectively
Each tissue derived from ten plants at the same growth stage were pooled together and surface sterilized with 70 % (v/v)ethyl alcohol for 1 min, 2%(v/v) NaOCl for 2 min, 95%(v/v) ethyl alcohol for 30 s and 30 % (v/v) H2O2 for 1 min, respectively