The simultaneous saccharification and fermentation (SSF) of pretreated sugar cane leavcs to produce
ethanol using a cellulolytic enzyme complex from Trichoderma reesei QM 9414 and Saccharomyces cerevisiae
NRRL-Y-132 was optimized. Enzymic saccharification parameters were evaluated prior to SSF studies. A
92% conversion of 2"5% substrate (alkaline hydrogen peroxide pretreated) to sugars was achieved at 50°C
and pH 4.5, using 7; reesei cellulase (40 FPU/g substrate), in 48 h. The pretreated substrate was then
subjected to an SSF process using the cellulase complex and S. cerevisiae cells. Optimization of the SSF
system is described. © 1998 Published by Elsevier Science Ltd. All rights reserved