Generally, the reaction rate increases with temperature while the stability of the enzymes declines, and at high temperature, catalyticactivity will be lost rapidly before significant conversion is reached(Garcia, Sanchez, Martinez, & Aracil, 1999; Foresti & Ferreira, 2007).
Temperature may affect the hydrolysis efficiency in a positive way or vice versa.
A rise in temperature will increase the reaction rate as explained by the transition state theory (Serri, Kamaruddin, & Long,2006). Normally, a high temperature used in the reaction causes the denaturation of enzyme. Thus the optimum temperature for hydrolysis of konjac powder by the enzyme was 50◦C.