The bottles were slowly moved from the pad section to the fan section (30% RH and 32±2 °C) in the greenhouse. The hardened plantlets were transferred to soil in black polybags for complete acclimatization. These were kept in a nursery covered with agro-net. The plants were then transferred to the field under the environmental conditions of Rajasthan. All the experiments were set up in completely randomized block design and repeated three times, each treatment having at least ten replicates. The observation on initiation, multiplication, maintenance, and regeneration of cultures was scored after a regular time interval. The rate of culture multiplication was measured by measuring fresh weight of callus at the time of subculture. The data were subjected to one-way ANOVA using SPSS 7.5 software.