Experiments were prepared by adding 10 vol.% inoculums to the
different media and statically incubating at 28 ◦C for 13 days. The
collected pellicles were washed with water and homogenized in
a Waring Blender for 5 min to obtain microfibrils. The microfibrils
were treated for 14 h in a 5 wt% KOH solution, rinsed until pH 7, and
homogenized in water for 5 min. The treatments and codification
of the cellulose obtained from different media are summarized in
Fig. 1.
Finally, films of constant thickness (0.1 mm) were prepared
from a suspension of different cellulose microfibrils (0.3 wt%) by
vacuum filtration and oven-dried at 70 ◦C for 48 h between glass
plates