4. Conclusion
These results show that L. fermentum CECT5716 resists the
process of microencapsulation in alginate beads with starch, and
the resultant microencapsulated probiotic preparation is stable at
4 C and, in a lesser extent, also at 20 C.
We have also shown a significant effect of starch and probiotics
in the microparticle size. The addition of the probiotic strain de-
creases the microparticle size in both formulations (with and
without starch), thus leading to a product suitable for application in
food.
In conclusion, ionic internal gelation technology with alginate
and starch seems to be a suitable procedure for protecting L. fer-
mentum and could allow the addition of this probiotic to food and
pharmaceutical preparations.
More studies are needed to elucidate whether the addition of
technological coadjuvants, such as, cryoprotectants, would be
suitable to obtain a good survival of the microencapsulated pro-
biotic through time. Futureworkswill also be aimed at studying the
functionality of the microencapsulating probiotic in a final matrix
and whether it improves survival through gastrointestinal tract.
4. ConclusionThese results show that L. fermentum CECT5716 resists theprocess of microencapsulation in alginate beads with starch, andthe resultant microencapsulated probiotic preparation is stable at4 C and, in a lesser extent, also at 20 C.We have also shown a significant effect of starch and probioticsin the microparticle size. The addition of the probiotic strain de-creases the microparticle size in both formulations (with andwithout starch), thus leading to a product suitable for application infood.In conclusion, ionic internal gelation technology with alginateand starch seems to be a suitable procedure for protecting L. fer-mentum and could allow the addition of this probiotic to food andpharmaceutical preparations.More studies are needed to elucidate whether the addition oftechnological coadjuvants, such as, cryoprotectants, would besuitable to obtain a good survival of the microencapsulated pro-biotic through time. Futureworkswill also be aimed at studying thefunctionality of the microencapsulating probiotic in a final matrixand whether it improves survival through gastrointestinal tract.
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