limited due to a low survival rate. Therefore, there is an urgent need to look for alternate means of propagation, which could ensure large-scale production of plants to fulfill the growing demands. In vitro propagation is a feasible alternative for the rapid multiplication and maintenance of germplasm. In vitro propagation ensures the production of true-to-type plants in limited time and space. The propagation from elite mature plants is preferred for this purpose as they are selected on past performances. This communication reports an efficient protocol for regeneration of plants of H. opposita using nodes derived from mature trees.