The significance of e (M1 cm1) is discussed in relation to mFRAP assay sensitivity, minimum detectable concentration,
and the dimensionless FRAP-value. Gallic acid showed 6.6 mol of Fe2+ equivalents compared to
2.3 mol of Fe+2 equivalents for ascorbic acid. Application of the mFRAP assay to Manuka honey samples
(rated 5+, 10+, 15+, and 18+ Unique Manuka Factor; UMF) showed that FRAP values (0.54–0.76 mmol Fe2+
per 100 g honey) were strongly correlated with UMF ratings (R2 = 0.977) and total phenols content
(R2 = 0.982)whilst the UMF rating was correlated with the total phenols (R2 = 0.999). In conclusion,
mFRAP assay results were successfully standardised to yield data corresponding to 1-cm spectrophotometer
which is useful for quality assurance purposes. The antioxidant capacity of Manuka honey was found
to be directly related to the UMF rating.
The significance of e (M1 cm1) is discussed in relation to mFRAP assay sensitivity, minimum detectable concentration,
and the dimensionless FRAP-value. Gallic acid showed 6.6 mol of Fe2+ equivalents compared to
2.3 mol of Fe+2 equivalents for ascorbic acid. Application of the mFRAP assay to Manuka honey samples
(rated 5+, 10+, 15+, and 18+ Unique Manuka Factor; UMF) showed that FRAP values (0.54–0.76 mmol Fe2+
per 100 g honey) were strongly correlated with UMF ratings (R2 = 0.977) and total phenols content
(R2 = 0.982)whilst the UMF rating was correlated with the total phenols (R2 = 0.999). In conclusion,
mFRAP assay results were successfully standardised to yield data corresponding to 1-cm spectrophotometer
which is useful for quality assurance purposes. The antioxidant capacity of Manuka honey was found
to be directly related to the UMF rating.
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