Approximately 0.1 g (Fw) of algae was ground in 10 mL of 100%
methanol and extracted at 4 °C in darkness for 24 h. This extract was
centrifuged at 5000 r/min for 10 min, and then the supernatant was
used to determine the contents of chlorophyll a (Chl a) and carotenoid
(Car) with an ultraviolet spectrophotometer (UV-1800; Shimadzu,
Kyoto, Japan). Chl a and Car concentrations were estimated according
to the methods described by Porrar (2002) and Parsons and Strickland
(1963), respectively.