From the results, an aeration rate of 300 mL/min and foam fractionation column height of 60 cm were optimal for foam fractionation. Using these conditions, one liter of diluted culture medium with palm oil and crude palm oil additions could be separated and concentrated to 167 and 200 mL of foamate containing 10.9 and 9.8 g/L of crude lipopeptide, respectively. Theselipopeptide yields were much higher than the integrated lipopep-tide production and recovery processes designed by Yeh et al.[5], which consisted of a conventional stirred tank fermenter con-nected with foam separation through its gaseous outlet column.Using 1.5 mL/min aeration rate and 300 rpm agitation, Yeh et al.[5] reported the highest lipopeptide concentration in foamate of6.45 g/L. The high lipopeptide yield could be due to the lower aer-ation and agitation rates used in this study, which resulted in lessshear force on the immobilized cells. Moreover, the sequentialproduction and recovery processes could reduce the risk of contam-inations in the fermenter compared to the integrated processes