A method to evaluate interactions b

A method to evaluate interactions between biologically active agents is presented. Synergism, zero interaction, and antagonism were easily detected with the three-dimensional approach proposed herein. This method is compatible with a checkerboard design to diagnose the interaction between agents and obviate the need to test their mixtures in a fixed concentration ratio as proposed by Chou and Talalay. Dose-response curves for individual agents were obtained, and experimental data fitted to appropriate equations by nonlinear regression. If zero interaction was present, the predicted effect could be calculated for each combination using the classical isobole equation with any spreadsheet having a command to solve mathematical equations by iteration. This allowed the selection of appropriate concentrations for the combination of two or more agents. Interaction between agents could be assessed in two ways: by comparing experimental with expected effects, if zero interaction is present; or by analyzing the reduction or increase in total dose found as a consequence of the interaction. The applicability of both approaches is discussed and, for purposes of comparison with other methods, examples based on published data are analyzed and commented upon.

ผลลัพธ์ (แอฟริกา) 1:

'N Metode om interaksies tussen biologies aktiewe agente evalueer word. Sinergisme, nul interaksie, en antagonisme is maklik opgespoor met die hierin voorgestelde drie-dimensionele benadering. Hierdie metode is versoenbaar is met 'n checker ontwerp om die interaksie tussen agente te diagnoseer en voorkom die behoefte om hul mengsels toets in 'n vaste verhouding konsentrasie as deur Chou en Talalay voorgestel. Dosis-responskurwes vir individuele agente is verkry, en eksperimentele data om toepaslike vergelykings toegerus deur lineêre regressie. As nul interaksie teenwoordig was, kon die voorspelde uitwerking bereken word vir elke kombinasie met behulp van die klassieke isobole vergelyking met enige spreadsheet met 'n opdrag om wiskundige vergelykings op te los deur iterasie. Dit het die keuse van geskikte konsentrasies vir die kombinasie van twee of meer agente. Interaksie tussen agente kan geassesseer word in twee maniere: deur dit te vergelyk met eksperimentele verwag effekte, as nul interaksie teenwoordig is; of deur die ontleding van die vermindering of toename in die totale dosis gevind as 'n gevolg van die interaksie. Die toepaslikheid van beide benaderings word bespreek en, vir doeleindes van vergelyking met ander metodes, is voorbeelde gebaseer op gepubliseerde data ontleed en daarop kommentaar gelewer word.