The volumetric yield of bacterial cellulose in hydrolysate was 2.25 g/L, which was 1.5-folds of that in waste water of candied jujube. The structures indicated that the fiber size distribution was 3–14 nm in those media with an average diameter beingaround 5.9 nm. The crystallinity index of BC from pretreatment medium was lower than that of with ou tpretreatment medium and BCs from various media had similar chemical binding. Ammonium citrate was a key factor for improving production yield and the crystallinity index of BC.