2.2.2. Fish
Fish (approximately 300 g ± 23 g, mean ± SD initial weight) were
stocked in tanks at 20 fish per 200-L-poly tanks. Water temperature
was maintained at 14 °C in a recirculating system. Lighting was maintained
on a 13:11 h diurnal cycle. Diets were randomly assigned to
three replicate tanks of fish. Fish were fed twice daily to apparent satiation
beginning 14 days prior to the first fecal collection.