Biomass yield of G. lucidum in submerged culture was investigated on account of its requirements for sources of carbon, nitrogen and the carbon : nitrogen (C:N) ratio. The study was carried out with the culture medium of potato dextrose broth enriched with the different carbon (glucose, maltose, starch) and nitrogen (aspartic acid, nitrogen nitrate, peptone) sources over a period of 15 days following standard procedures. The highest biomass of 460±89mg was recorded in the broth medium incorporated with maltose which is significantly different (p