Concentration of carbon and nitrogen source was main effect on lipid accumulation by oleaginous
yeast. In case of T. asahii, it could assimilate molasses and (NH4)2SO4 for lipid production which an optimal level of molasses and (NH4)2SO4 were 12% and 0.5 g/l, respectively. These indicating that it is possible to produce single cell oil by T. asahii for use as unsaturated fatty acid source in dairy cow diet.