Adipogenic Potential of Orbital ASCs. We tested the ability of
orbitally derived ASCs to differentiate in adipocytes using previously
described methods.10 Nasal and central ASCs were incubated in either
control media or adipogenic differentiation media for 2 weeks and stained
with oil red O to determine lipid accumulation. Figure 2 shows accumulation
of lipid as evidenced by oil red O staining when ASCs are incubated
with adipogenic differentiation media (right panel). There was no oil red O
staining when ASCs were cultured in control media (left panel). Quantitatively,
the percent positive oil red O staining cells ranged from 11% to