Fig. 4. Effect of ultra-sonication (0, 30 s, 2 min and 4 min) on pasteurised whole milk fat globule size (A) and on ANGPTL4 mRNA level of HCT 116 cells after 6 h exposure at 10% (B). A commerical whole milk (Comm.) was used for comparison with the control without added milk. Results are shown as means ± SD (n ¼ 3); different letters indicate significant different values between treatments (P < 0.05).