H1 nuclear magnetic resonance (NMR) spectroscopy has recently been used to identify Acanthamoeba in vitro by Hauber et al. [29]. By profiling the biochemical signature of different strains of the organism, it is anticipated that this method could yield a high level of sensitivity and specificity as a diagnostic test. Its application would be similar to PCR testing, so further study is needed to see how it compares to PCR in terms of diagnostic accuracy and time efficiency.