The experiment was conducted on 15 mature males of Green-legged Partridge breed kept individually in cages (60×50×75 cm) at 18–20 °C, under 14L:10D photoperiod. Birds were fed with commercial feed for breeding flocks, without additional antioxidant supplementation. Water was providedad libitum. The pooled semen was collected twice a week, by the dorso-abdominal massage method[12]. The collection was always performed by the same people and under the same conditions. Only clean ejaculates were used for further analysis.