Examination of R. planticola biofilm viability on polymers such
as PET (polyethylene terephtalate) fibers was performed using
CSLM analysis. R. planticola bacterial cells were grown in MMA in
the presence of PET fibers, after 72 h the substratum was washed
with phosphate buffered saline, fluorescently stained using a Live/
Dead Kit L7007 (Molecular Probes, Israel) and examined using
CSLM (Zeiss LSM 700).