Thus, a new method for glucose detection
was built on these bases consequently. The sensor for glucose has a
(a)
Wavelength / nm
500 550 600 650 700
RTP intensity
0
100
200
300
400
500
600
0
0.8 mM
[Glucose]
[Glucose] / mM
0.0 0.2 0.4 0.6 0.8 1.0
RTP
0
50
100
150
200
250
(b) 300
0.00 0.04 0.08 0.12
0
40
80
120
160 0.0 0.1 0.2 0.3 0.4 0.5
60
120
180
240
300
Fig. 5. (a) Glucose concentration-dependent RTP emission of the MPA-capped Mn-doped ZnS QDs. (b) Plots of DRTP as a function of glucose concentration show two linear
ranges. Buffer, 10 mM PBS (pH 7.4); MPA-capped Mn-doped ZnS QDs, 40 mg L1
; GOD, 500 U L1
.
Table 1
Comparison of performances among nanomaterials-based glucose sensing methods.
Nanomaterials Enzyme immobilization Detection scheme Detection range LOD References
CdTe QDs Not needed Fluorescence 1.0 lM to 0.5 mM and 1–20 mM 0.1 lM [37]
Ag/CNT/Ch film LbL assembly Amperometry 0.5–50 lM 0.1 lM [40]
CdTe film LbL assembly Fluorescence 0.5–16 mM 0.5 mM [15]
CdTe QDs Not needed Fluorescence 1 lM to 0.15 mM and 0.15–1 mM 0.01 lM [38]
CdTe/CdS QDs Not needed Fluorescence 1.8 lM to 1 mM and 2–30 mM 1.8 lM [41]
Mn-doped ZnS QDs Covalent conjugation Phosphorescence 10 lM to 0.1 mM and 0.1–1 mM 3 lM [18]
Mn-doped ZnS QDs Not needed Phosphorescence 8.8 lM to 0.1 mM and 0.1–0.8 mM 2.9 lM This work
Wavelength / nm
350 400 450 500 550
Fluorescence intensity
0
100
200
300
400
500
RTP intensity
0
100
200
300
400
500
3
1 and 2
4
Fig. 6. The RTP and fluorescence spectra of urine (curves 1, 3) and serum (curves 2,
4).
Table 2
Recovery for the determination of glucose in urine and serum samples (Mean ± s;
n = 3).
Type of samples Glucose spiked (mM) Recovery (%)
Human urine 0.05 102 ± 5
0.2 98 ± 3
0.4 95 ± 3
Human serum 0.05 97 ± 4
0.2 101 ± 2
0.4 96 ± 4
Y. Miao / Sensing and Bio-Sensing Research 5 (2015) 112–116 115
detection limit of 0.0029 mM and two linear ranges from 0.005 to
0.1 mM and from 0.1 to 0.4 mM. Combined the advantage of other
nanomaterials-based glucose detection methods, which including
simple operation, high sensitivity and no interference from the
background fluorescence of biological fluids, the glucose detection
ability was improved, which further certificated that this method
can be used to detect the glucose content in body fluid.