The untreated flank (right flank) of treated and control animals were clipped free of hair
(closely-clipped). An occlusive patch, separately containing the appropriate amount of
test substance and positive control substance were applied to the untreated flanks previously
mentioned. The same manner as those in Day 0 - treatment group and Day 0 – positive control group were performed. Skin reactions were evaluated and recorded at 24 and 48 hrs after the patch application.