บทคัดย่อAbstract: Toxicity-data of two carbamate insecticides, carbaryl and carbofuran, and three fungicides,
และสามเชื้อราziram, zineb ziram, zineb and mancozeb with rice-field N2-fixing cyanobacterium Cylindrospermum sp., obtained by
ที่ได้จากการเพาะเลี้ยงเนื้อเยื่อเจริญเติบโตและการที่อินเตอร์เฟซในดินน้ำมาวิเคราะห์โดยวิธี การเสริมสร้างการเจริญเติบโตของความเข้มข้นของความเข้มข้นที่มีประสิทธิภาพไม่สังเกตเห็นความเข้มข้นต่ำสุดที่สูงที่สุดมีความเข้มข้นและอนุญาตconcentration100 in vitro growth and at soil-water interface, were analyzed by the probit method. Growth enhancing
concentration, no-observed effective concentration, minimum inhibitory concentration, the highest
permissive concentration and lethal concentration100 (LC100) were determined experimentally. The LC50
values of carbaryl, carbofuran, ziram, zineb and mancozeb in N2-fixing liquid medium were 56.2, 588.8,
0.07, 4.2 and 3.4 μg/mL, respectively, whereas the corresponding LC100 values were 100.0, 1500.0,
0.17, 25.0 and 9.0 μg/mL, respectively. The LC50 values of these pesticides in succession in N2-fixing
agar medium were 44.7, 239.9, 0.07, 1.8 and 2.3 μg/mL, respectively, whereas the corresponding LC100
values were 100.0, 600.0, 0.17, 10.0 and 7.0 μg/mL, respectively. Similar results with nitrate
supplemented liquid and agar media indicated that nitrate supplementation had toxicity reducing effect.
The LC50 and LC100 values of toxicity in the N2-fixing liquid medium at soil-water interface were 91.2 and
200.0 μg/mL for carbaryl, 2 317 and 6 000 μg/mL for carbofuran, 0.15 and 0.50 μg/mL for ziram, 16.4
and 50.0 μg/mL for zineb, and 7.2 and 25.0 μg/mL for mancozeb, respectively. Each LC100 value at soilwater
interface with a pesticide was significantly higher than its corresponding LC100 value at liquid/agar
media. It can be concluded that, under the N2-fixing conditions, the cyanobacterium tolerated higher
levels of each pesticide at soil-water interface.
Key words: carbamate pesticide; Cylindrospermum sp.; cyanobacterium; lethal concentration; probit
analysis; soil-water interface
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