he 2,2-diphenyl-2-picrylhydrazyl (DPPH) was used to determine
the free radical scavenging activity of a propolis
sample. 1 ml of ethanol solution and 0.1 mM of DPPH in
methanol was added to 2 ml of the sample from MAE
propolis extract [3]. The control measurement was prepared
from 1 ml ethanolic solution with 70% ethanol and 2 ml of
methanolic DPPH solution. The mixture was mixed and left
in dark for 30 min. The absorbance was measured at 517
nm while the ethanolic solution with 70% ethanol was used
as blank. The ability of a propolis sample to scavenge DPPH
radicals was calculated using the following equation:
The effective concentration that is able to scavenge 50%
of the DPPH radicals is then calculated with the construction
of dose–response graph. The analyses were done in
triplicates (n = 3). In addition, the significant difference of
mean values for yield, total phenolic content, total flavonoids
content and free radical scavenging activity under
various factors obtained in this work were compared
using one-way analysis of variance (ANOVA).