It has been suggested that cutting promotes the transfer
of endogenous hormones to the wounded area, enhancing
the morphogenesis ability of the cells (Park & Son 1988). But
our study revealed no advantages for any cutting mode for
in vitro regeneration of shoots from internodal explants of
Thomson navel sweet orange. The results of the present
study indicated that there are no differences between ILC
and ITC in terms of explant response and developed shoots
per explant (Table 1). Moreover, they were statistically simi-
lar considering the response promptness (Fig. 1).
The shoots produced from Shoot tips or internodal seg-
ments (both ITC and ILC) were morphologically different at
the initiation stage. The shoots from internodal segments
were green and thicker, while shoots derived from shoot tips
were pale green and somehow etiolated with some callusing
at the base (Fig. 3). These morphological variations reduced
when the shoots grew further.