The lyophilized
powder was then homogenised, and 0.3 g were treated with
3 mL of a water:ACN mixture (7:3, v/v) containing a 5% (v/v) Celluclast
1.5L enzyme at pH 5.0, sonicated at 55 C for 15 min using an
ultrasound power of 0.5 W/mL, and centrifuged at 10,000g for
10 min.