2.3. Preparation of extracts
Dried spices were grounded using mixer grinder (Preethi ChefPro
model, Indianmake) and sievedwell using vertical vibratory sieve shaker
(Labortechnik Gmbh, Ilmenau) for 20 min. Spice powders (500 g)
were extracted with water using soxhlet apparatus at a temperature
of 80 °C for 5 h. The extracts were filtered through whatman filter
paper no.1 (Whatman International, Ltd.,) and the extract was lyophilized
using a freeze-dryer. Finally spice extracts were dissolved in
water in the ratio of 1:10 (w/v) for further studies.