The total phenolics content in the macaroni (control) and MPP
incorporated macaroni was estimated by the Folin–Ciocalteau method (Swain & Hillis, 1959) using gallic acid as standard. The absorbance of the color formed was measured at 725 nm using a spectrophotometer and the results were expressed as gallic acid equivalents (GAE). The total carotenoid content was estimated according to themethod described by Litchenthaler (1987) using following equations and the total carotenoids obtained from this equation is microgram per ml of extract.