2.5. Analysis
2.5.1. HPLC analysis of capsaicinoids
The samples of capsaicinoids were analyzed by highperformance
liquid chromatography (HPLC) on Agilent 1200 HPLC
system composed of a quaternary pump with a degasser, a thermostatted
column compartment, a detector of a UV-detector (a
variable wavelength detector, VWD) and an autosampler and 1200
ChemStation software. The column used was an Agilent Eclipse
XDB-C18 column (250 mm 4.6 mm I.D., 5 mm), the mobile phase
was methanolewater (65:35, v/v) with flowrate at 0.8 mL/min. The
wavelength of UV detector was set at 280 nm and column temperaturewas
set at 35 C. All samples were filtered through 0.45 mm
membrane before HPLC analysis and the injection volume of sample
was 20 mL.