1-MCPAND STORAGE TREATMENTS. After fruit were sealed in the
plastic barrels, concentrated 1-MCP gas was injected into the
barrel to bring the final gas concentration to 0.7 μL·L–1, which was
expected to be sufficient to saturate the response to 1-MCP (Fan
et al., 1999). To create the concentrated 1-MCP gas, 2.5 g of
EthylBloc was placed in a 1-L glass jar. The jar was sealed with
a lid fitted with a rubber serum stopper. Fifty milliliters of dilute
KOH buffer was added to the jar by injecting the buffer solution
through the lid. The 1-MCP was allowed to release for at least 3
h. Fifty milliliters of the headspace in the glass jar was removed
using a 50-mL syringe and injected into the sealed barrels. As
each 50 mL volume of gas was removed, 50 mL of water was
injected into the jar, maintaining a slight positive pressure in the
container and preventing dilution of 1-MCP in the jar headspace
1-MCPAND STORAGE TREATMENTS. After fruit were sealed in theplastic barrels, concentrated 1-MCP gas was injected into thebarrel to bring the final gas concentration to 0.7 μL·L–1, which wasexpected to be sufficient to saturate the response to 1-MCP (Fanet al., 1999). To create the concentrated 1-MCP gas, 2.5 g ofEthylBloc was placed in a 1-L glass jar. The jar was sealed witha lid fitted with a rubber serum stopper. Fifty milliliters of diluteKOH buffer was added to the jar by injecting the buffer solutionthrough the lid. The 1-MCP was allowed to release for at least 3h. Fifty milliliters of the headspace in the glass jar was removedusing a 50-mL syringe and injected into the sealed barrels. Aseach 50 mL volume of gas was removed, 50 mL of water wasinjected into the jar, maintaining a slight positive pressure in thecontainer and preventing dilution of 1-MCP in the jar headspace
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