Growth of the bacteria is the primary requirement to carry out biodegradation. The microbial population
directly accounts the production of biosurfactant accountable for aiding the hydrocarbon degradation. Figure 2
shows the growth of Bacillus subtilis on waxy crude oil at room temperature. The biomass dry weight reaches 2.11
g/L when the CFU reaches the maximum, and the steady state was achieved further. It is inferred that the growth