A group of 1050 male goldfish measuring 51.20 2.21 g
and 14.22 1.37 cm was used for the broodstock–conditioning
experiment with the carotenoid-enriched diet. The
experiment lasted for 150 days, and it compared the diets
enriched with synthetic astaxanthin (Carophyll Pink 10%;
DSM Nutritional Products Philippines, Inc.dBright Science,
Brighter Living, the Netherlands) and b-carotene (b-carotene
10% CWS; Direct Food Ingredients Ltd., England) with
a control diet for a total of seven treatments run in
triplicate.