a-1) E. coli DH5α strain was used to evaluate the study
conducted for the detection of E. coli using specific primers.
a-2) CRM04 and CRM07: CRM04 (QCMIC-001-04) contains 83.00
依CFU/100 mL of coliform bacteria except E. coli, and CRM007
(QCMIC-007) contains (100.00依2.00) CFU/mL of coliform bacteria
and E. coli.
a-3) Synthetic samples: 10 samples containing isolated
bacteria in 250 mL distilled water.
a-4) Isolated bacteria from contaminated water: Three
bacterial strains were isolated from positive most probable
number (MPN) samples and then purified. They were
identified using microbiological techniques (up to the level of
identification of species). Therefore, mediums such as nutrient
agar, EMB, IMVIC phenylalanine and urea were used in addition
to Gram stain.