In order to identify the relevant parameters that could contribute
to the sensitivity of the proposed method, two screening
23 full factorial designswere carried out, both with three replicates
in the central body being in this way, able to quantify the experimental
error [21]. Regarding the solvents, cyclohexane was placed
at the central point since it is an intermediate polar compound, in
relation to isooctane and toluene. In the first factorial design, the
investigated factors and their levelswere selected after preliminary
experimental studies. In turn, the second factorial design aimed to
better optimize the initial parameters to reach the best possible
working conditions.