However, lipid oxidation was not measured in these slices. Thus, in follow-up work we examined metmyoglobin formation and lipid oxidation in longissimus slices during 30h of storage in 1% O2. The results from two trials are
presented in Figure 3. In brief, Holstein steers (n = 3 per treatment per trial) were nominally supplemented
with a target dose of 0, 500, or 2,000 mg a-tocopheryl acetate·steer-1·d-1 for 125 d. This resulted in muscle
a-tocopherol concentrations of 1.03, 3.76, and 7.39 mg a-tocopherol/g tissue, respectively. Strip loins were
removed at 24 h postmortem, vacuum-packed shipped to the University of Connecticut Meat Laboratory, and
stored at 4°C. At 14 d postmortem, meat cores were prepared from longissimus slices (1 cm) and wrapped beef cores were analyzed for lipid oxidation using a
thiobarbituric acid reactive substances (TBARS)
assay
However, lipid oxidation was not measured in these slices. Thus, in follow-up work we examined metmyoglobin formation and lipid oxidation in longissimus slices during 30h of storage in 1% O2. The results from two trials are
presented in Figure 3. In brief, Holstein steers (n = 3 per treatment per trial) were nominally supplemented
with a target dose of 0, 500, or 2,000 mg a-tocopheryl acetate·steer-1·d-1 for 125 d. This resulted in muscle
a-tocopherol concentrations of 1.03, 3.76, and 7.39 mg a-tocopherol/g tissue, respectively. Strip loins were
removed at 24 h postmortem, vacuum-packed shipped to the University of Connecticut Meat Laboratory, and
stored at 4°C. At 14 d postmortem, meat cores were prepared from longissimus slices (1 cm) and wrapped beef cores were analyzed for lipid oxidation using a
thiobarbituric acid reactive substances (TBARS)
assay
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