2.1. Study settings
This study was conducted at both the University of Chicago and the
Institute for Advanced Medical Research and Training (IAMRAT) at
Ibadan. It involved online IHC training sessions. The study was
approved by institutional review boards of the coordinating institutions in Ibadan and the University of Chicago[10]. After an initial
training (stage 1), the first performance evaluation (stage 2) was
conducted followed by a review of process and then a session of
online training and discussion (stage 3) and a second evaluation of
performance (stage 4). Statistical analysis was performed with
StataSE software, version 10 (STATA, Cary, North Carolina). Theκ
statistic served to test inter-tester agreement in IHC staining and
scoring. Overall inter-tester agreement was obtained based on κ
coefficient calculated using a 4 × 4 weighting matrix