The proposed separation system was applied to determine SCFAs in samples of leachate from a municipal landfill. An exemplary chromatogram is given in Fig. 4, and the level of the SCFAs in the landfill leachate ranged from 0.45 to 15 mg L−1. The contents of particular acids are presented as average of 4 measurements ± extended uncertainty for significance level α = 0.05 and caluculated as .; for n = 4 and α = 0.05, k equals 2. Ethanoic acid was at the highest concentration of 15.2 ± 0.73 mg L−1 and the second highest was propanoic acid (4.5 ± 0.19 mg L−1). The other analytes were 2-methylpropanoic acid (1.21 ± 0.061 mg L−1), butanoic acid (2.12 ± 0.074 mg L−1), 3-methylbutanoic acid (0.48 ± 0.077 mg L−1) and pentanoic acid (0.45 ± 0.059 mg L−1). Hexanoic, heptanoic and octanoic acids were below LOQ.