The present work reported first investigations on fumigant and anti-cholinesterase activities of C. auran-tium essential oils and pure limonene towards Tunisian strain of Bemisia tabaci. GC and GC–MS analysisshowed a high percent of limonene (87.523%). C. aurantium oils and the single compound were knownto be potent AChE inhibitor with IC50value of 2.94 mM and 3.54 mM, respectively. Furthermore, it wasobserved that the oil and its major compound showed a reversible non-competitive inhibition revealedthat these components might bind both the enzyme alone and the enzyme–substrate. Significant pestfumigant activity was demonstrated. Fumigant treatments have shown that B. tabaci mortality rateincreased with the increase in essential oils concentration. Complete insect mortality was detected at thehighest concentration (20 L/L air) within 24 h of exposure, whereas, at the least concentration (2.5 L/Lair) mortality varied from 41 to 47.67% for the single compound and the oil, respectively. These resultssuggest that oils from C. aurantium peels may be promising as models to develop new insecticides thatmight be applied into the integrated management of whiteflies.