88). Prior to N-terminal sequencing an additional puri®cation step was
included. Toxins were applied to a RP C8 column (Applied Biosystems),
equilibrated in 0.1% tri¯uoroacetic acid in water. Elution was performed with a
30 min linear gradient to 80% (v/v) acetonitrile, 0.1% tri¯uoroacetic acid in water
at a ¯ow rate of 1 ml/min on a Waters HPLC system. The eluted proteins were