results (data not shown) provided information about optimized
media composition, the general behaviour of Ps. chlororaphis in
presence of biodiesel as carbon source,the most efficient emulsifier
(Grinsted Citrem SP70) and the toxicity of different concentrations
of saturated biodiesel. Not only that the strain adapted really fast to
saturated biodiesel as sole carbon source, there were no observable
inhibiting effects regarding the applied biodiesel concentrations in
the investigated range (1 to 15 g/L). Based on these promising data,
the conditions for the mcl-PHA biosynthesis on bioreactor scale
were chosen