increased two fold in a one week culture when compared
to that in the unelicited Rubia akane Nakai cell culture
(Jin et al., 1999). In addition, chitosan elicitor was also
found to enhance secondary metabolite production in
Sorbus aucuparia cell cultures (Gaid et al., 2009) and
Brugmansia candida hairy root cultures (Pitta-Alvarez
and Giulietti, 1999). In the present study alkaloid
accumulation in the media culture was also investigated
(Table 2a-b). It was found that chitosan forced the