2.4. Vitamins
2.4.1. Vitamin C content
Vitamin C in fresh milk was determined by titrimetric method
(Bosset and Eberhard, 1991). Ten grams of fresh milk sample
was mixed with 20 mL of 10% Meta phosphoric acid and
40 mL of distilled water at 50 C was added into a 100 mL long
neck amber glass flask and was set on an agitator for 5 min.
After that, the volume of the resultant adduct was made up
to the mark with distilled water and collected filtrate in a conical flask. Ten milliliters of filtrate was mixed with 5 mL of
10% acetic acid and 10 mL of 2% Meta phosphoric acid in
a beaker. It was titrated against 2,6-dichlorophenolindophenol
indophenol sodium salt dihydrate and the volume used was
noted to determine vitamin C contents by the following
formula.